| Enzyme | Restriction site | Origin | Temperature |
|---|
| OliI | ...CACNN NNGTG...
...GTGNN NNCAC... | Oceanospirillum linum | 37 oC |
| PacI | ...TTAAT TAA...
...AAT TAATT... | Pseudomonas alcaligenes | 37 oC |
| PaeI | ...GCATG C...
...C GTACG... | Pseudomonas aeruginosa | 37 oC |
| PaeR7I | ...C TCGAG...
...GAGCT C... | Pseudomonas aeruginosa | 37 oC |
| PagI | ...T CATGA...
...AGTAC T... | Pseudomonas alcaligenes | 37 oC |
| PasI | ...CC CWGGG...
...GGGWC CC... | Pseudomonas anguilliseptica | 55 oC |
| PauI | ...G CGCGC...
...CGCGC G... | Paracoccus alcaliphilus | 37 oC |
| PceI | ...AGG CCT...
...TCC GGA... | Planococcus citreus | 50 oC |
| PciI | ...A CATGT...
...TGTAC A... | Planococcus citreus | 37 oC |
| PctI | ...GAATGCN N...
...CTTAC GNN... | Planococcus citreus | 37 oC |
| PdiI | ...GCC GGC...
...CGG CCG... | Brevundimonas diminuta | 37 oC |
| PdmI | ...GAANN NNTTC...
...CTTNN NNAAG... | Brevundimonas diminuta | 37 oC |
| PfeI | ...G AWTC...
...CTWA G... | Psychrobacter faecalis | 37 oC |
| PflFI | ...GACN NNGTC...
...CTGNN NCAG... | Pseudomonas fluorescens | 37 oC |
| PflMI | ...CCANNNN NTGG...
...GGTN NNNNACC... | Pseudomonas fluorescens | 37 oC |
| PfoI | ...T CCNGGA...
...AGGNCC T... | Pseudomonas fluorescens | 37 oC |
| PhoI | ...GG CC...
...CC GG... | Pyrococcus horikoshii | 75 oC |
| PinAI | ...A CCGGT...
...TGGCC A... | Pseudomonas inaequalis | 37 oC |
| Ple19I | ...CGAT CG...
...GC TAGC... | Paucimonas lemoignei | 37 oC |
| PleI | ...GAGTCNNNN NN...
...CTCAGNNNNN N... | Paucimonas lemoignei | 37 oC |
| PmaCI | ...CAC GTG...
...GTC CAC... | Stenotrophomonas maltophilia | 37 oC |
| PmeI | ...GTTT AAAC...
...CAAA TTTG... | Pseudomonas mendocina | 37 oC |
| PmlI | ...CAC GTG...
...GTG CAC... | Stenotrophomonas maltophilia | 37 oC |
| PpsI | ...GAGTCNNNN NN...
...CTCAGNNNNN N... | Pseudomonas pseudoalcaligenes | 37 oC |
| PpuMI | ...RG GWCCY...
...YCCWG GR... | Pseudomonas putida | 37 oC |
| PscI | ...A CATGT...
...TGTAC A... | Paracoccus species | 30 oC |
| PshAI | ...GACNN NNGTC...
...CTGNN NNCAG... | Plesiomonas shigelloides | 25 oC |
| PshBI | ...AT TAAT...
...TAAT TA... | Plesiomonas shigelloides | 37 oC |
| PsiI | ...TTA TAA...
...AAT ATT... | Pseudomonas species | 37 oC |
| Psp124BI | ...GAGCT C...
...C TCGAG... | Pseudomonas species | 37 oC |
| Psp1406I | ...AA CGTT...
...TTGC AA... | Pseudomonas pseudoalcaligenes | 37 oC |
| Psp5II | ...RG GWCCY...
...YCCWG GR... | Pseudomonas species | 37 oC |
| Psp6I | ...N CCWGGN...
...NGGWCC N... | Pseudomonas species | 55 oC |
| PspCI | ...CAC GTG...
...GTG CAC... | Pseudomonas species | 37 oC |
| PspEI | ...G GTNACC...
...CCANTG G... | Pseudomonas species | 37 oC |
| PspGI | ...N CCWGGN...
...NGGWCC N... | Pyrococcus species | 75 oC |
| PspLI | ...C GTACG...
...GCATG C... | Pseudomonas species | 37 oC |
| PspN4I | ...GGN NCC...
...CCN NGG... | Pseudomonas species | 37 oC |
| PspOMI | ...G GGCCC...
...CCCGG G... | Pseudomonas species | 37 oC |
| PspPPI | ...RG GWCCY...
...YCCWG GR... | Pseudomonas species | 37 oC |
| PspXI | ...VC TCGAGB...
...BGAGCT CV... | Pseudomonas species | 37 oC |
| PsrI | ...NNNNNN NNNNNNNGAACNNNNNNTACNNNNNNNNNNNN N...
...N NNNNNNNNNNNNCTTGNNNNNNATGNNNNNNN NNNNNN... | Pseudomonas stutzeri | 30 oC |
| PstI | ...CTGCA G...
...G ACGTC... | Providencia stuartii | 37 oC |
| PsuI | ...R GATCY...
...TCTAG R... | Providencia stuartii | 37 oC |
| PsyI | ...GACN NNGTC...
...CTGNN NCAG... | Pseudomonas syringae | 37 oC |
| PvuI | ...CGAT CG...
...GC TAGC... | Proteus vulgaris | 37 oC |
| PvuII | ...CAG CTG...
...GTC GAC... | Proteus vulgaris | 37 oC |
