| Enzyme | Restriction site | Origin | Temperature |
|---|
| AarI | ...CACCTGCNNNN NNNNN...
...GTGGACGNNNNNNNN N... | Arthrobacter aurescens | 37 oC |
| AasI | ...GACNNNN NNGTC...
...CTGNN NNNNCAG... | Arthrobacter aurescens | 37 oC |
| AatII | ...GACGT C...
...C TGCAG... | Acetobacter aceti | 37 oC |
| AccI | ...GT MKAC...
...CAKM TG... | Acinetobacter calcoaceticus | 37 oC |
| AccII | ...CG CG...
...GC GC... | Acinetobacter calcoaceticus | 37 oC |
| AccIII | ...T CCGGA...
...AGGCC T... | Acinetobacter calcoaceticus | 65 oC |
| Acc16I | ...TGC GCA...
...ACG CGT... | Acinetobacter calcoaceticus | 37 oC |
| Acc36I | ...ACCTGCNNNN NNNNN...
...TGGACGNNNNNNNN N... | Acinetobacter calcoaceticus | 37 oC |
| Acc65I | ...G CTACC...
...CCATG G... | Acinetobacter calcoaceticus | 37 oC |
| AccB1I | ...G GYRCC...
...CCRYG G... | Acinetobacter calcoaceticus | 37 oC |
| AccB7I | ...CCANNNNN TGG...
...GGTN NNNNACC... | Acinetobacter calcoaceticus | 37 oC |
| AccBSI | ...CCG CTC...
...GGC GAG... | Acinetobacter calcoaceticus | 37 oC |
| AciI | ...C CGC...
...GGC G... | Arthrobacter citreus | 37 oC |
| AclI | ...AA CGTT...
...TTGC AA... | Acinetobacter calcoaceticus | 37 oC |
| AclWI | ...GGATCNNNN NN...
...CCTAGNNNNN N... | Acinetobacter calcoaceticus | 37 oC |
| AcsI | ...R AATTY...
...YTTAA R... | Arthrobacter citreus | 50 oC |
| AcuI | ...CTGAAGNNNNNNNNNNNNNNNN N...
...GACTTCNNNNNNNNNNNNNN NNN... | Acinetobacter calcoaceticus | 37 oC |
| AcvI | ...CAC GTG...
...GTG CAC... | Aeromonas caviae | 37 oC |
| AcyI | ...GR CGYC...
...CYGC RG... | Anabaena cylindrica | 50 oC |
| AdeI | ...CACNNN GTG...
...GTG NNNCAC... | Alcaligenes denitrificans | 37 oC |
| AfaI | ...GT AC...
...CA TG... | Acidocella facilis | 37 oC |
| AfeI | ...AGC GCT...
...TCG CGA... | Alcaligenes faecalis | 37 oC |
| AflII | ...C TTAAG...
...GAATT C... | Anabaena flos-aquae | 37 oC |
| AflIII | ...A CRYGT...
...TGYRC A... | Anabaena flos-aquae | 37 oC |
| AgeI | ...A CCGGT...
...TGGCC A... | Ruegeria gelatinovorans | 37 oC |
| AhdI | ...GACNNN NNGTC...
...CTGNN NNNCAG... | Aeromonas hydrophila | 37 oC |
| AhlI | ...A CTAGT...
...TGATC A... | Pseudoalteromonas haloplanktis | 37 oC |
| AjnI | ...N CCWGGN...
...NGGWCC N... | Acinetobacter johnsonii | 55 oC |
| AleI | ...CACNN NNGTG...
...GTGNN NNCAC... | Microbacterium liquefaciens | 37 oC |
| AluI | ...AG CT...
...TC GA... | Arthrobacter luteus | 37 oC |
| Alw21I | ...GWGCW C...
...C WCGWG... | Acinetobacter lwoffii | 37 oC |
| Alw26I | ...GTCTCN NNNNN...
...CAGAGNNNNN N... | Acinetobacter lwoffii | 37 oC |
| Alw44I | ...G TGCAC...
...CACGT G... | Acinetobacter lwoffii | 37 oC |
| AlwI | ...GGATCNNNN NN...
...CCTAGNNNNN N... | Acinetobacter lwoffii | 37 oC |
| AlwNI | ...CAGNNN CTG...
...GTC NNNGAC... | Acinetobacter lwoffii | 37 oC |
| Ama87I | ...C YCGRG...
...GTGCY C... | Alteromonas macleodii | 37 oC |
| Aor13HI | ...T CCGGA...
...AGGCC T... | Acidiphilium organovorum | 37 oC |
| Aor51HI | ...AGC GCT...
...TCG CGA... | Acidiphilium organovorum | 37 oC |
| ApaI | ...GGGCC C...
...C CCGGG... | Acetobacter pasteurianus | 37 oC |
| ApaLI | ...G TGCAC...
...CACGT G... | Acetobacter pasteurianus | 37 oC |
| ApeKI | ...G CWGC...
...CGWC G... | Aeropyrum pernix | 37 oC |
| ApoI | ...R AATTY...
...YTTAA R... | Arthrobacter protophormiae | 50 oC |
| AscI | ...GG CGCGCC...
...CCGCGC GG... | Arthrobacter species | 37 oC |
| AseI | ...AT TAAT...
...TAAT TA... | Aquaspirillum serpens | 37 oC |
| AsiSI | ...GCGAT CGC...
...CGC TAGCG... | Arthrobacter species | 37 oC |
| AspI | ...GACN NNGTC...
...CTGNN NCAG... | Achromobacter species | 37 oC |
| Asp700I | ...GAANN NNTTC...
...CTTNN NNAAG... | Achromobacter species | 37 oC |
| Asp718I | ...G GTACC...
...CCATG G... | Achromobacter species | 37 oC |
| AspA2I | ...C CTAGG...
...GGATC C... | Achromobacter species | 37 oC |
| AspEI | ...GACNNN NNGTC...
...CTGNN NNNCAG... | Achromobacter species | 37 oC |
| AspHI | ...GWGCW C...
...C WCGWG... | Achromobacter species | 37 oC |
| AspLEI | ...GCG C...
...C GCG... | Achromobacter species | 37 oC |
| AspS9I | ...G GNCC...
...CCNG G... | Achromobacter species | 37 oC |
| AssI | ...AGT ACT...
...TCA TGA... | Arthrobacter species | 37 oC |
| AsuII | ...TT CGAA...
...AAGC TT... | Anabaena subcylindrica | 37 oC |
| AsuC2I | ...CC SGG...
...GGS CC... | Actinobacillus suis | 37 oC |
| AsuHPI | ...GGTGANNNNNNNN N...
...CCACTNNNNNNN NN... | Actinobacillus suis | 37 oC |
| AsuNHI | ...G CTAGC...
...CGATC G... | Actinobacillus suis | 37 oC |
| AvaI | ...C YCGRG...
...GRGCY C... | Anabaena variabilis | 37 oC |
| AvaII | ...G GWCC...
...CCWG G... | Anabaena variabilis | 37 oC |
| AviII | ...TGC GCA...
...ACG CGT... | Anabaena variabilis | 37 oC |
| AvrII | ...C CTAGG...
...GGATC C... | Anabaena variabilis | 37 oC |
| AxyI | ...CC TNAGG...
...GGANT CC... | Gluconacetobacter xylinus | 37 oC |
