| Enzyme | Restriction site | Origin | Temperature |
|---|
| Cac8I | ...GCN NGC...
...CGN NCG... | Clostridium acetobutylicum | 37 oC |
| CaiI | ...CAGNNN CTG...
...GTC NNNGAC... | Delftia acidovorans | 60 oC |
| CciNI | ...GC GGCCGC...
...CGCCGG CG... | Curtobacterium citreum | 65 oC |
| CelII | ...GC TNAGC...
...CGANT CG... | Coccochloris elabens | 65 oC |
| CfoI | ...GCG C...
...C GCG... | Clostridium formicoaceticum | 37 oC |
| Cfr9I | ...C CCGGG...
...GGGCC C... | Citrobacter freundii | 37 oC |
| Cfr10I | ...R CCGGY...
...TGGCC R... | Citrobacter freundii | 37 oC |
| Cfr13I | ...G GNCC...
...CCNG G... | Citrobacter freundii | 37 oC |
| Cfr42I | ...CCGC GG...
...GG CGCC... | Citrobacter freundii | 37 oC |
| CfrI | ...Y GGCCR...
...RCCGG Y... | Citrobacter freundii | 65 oC |
| ClaI | ...AT CGAT...
...TAGC TA... | Caryophanon latum | 65 oC |
| CpoI | ...CG GWCCG...
...GCCWG GC... | Corynebacterium variabile | 55 oC |
| CseI | ...GACGCNNNNN NNNNNN...
...CTGCGNNNNNNNNNN N... | Chryseobacterium species | 65 oC |
| CspI | ...CG GWCCG...
...GCCWG GC... | Clostridium species | 60 oC |
| Csp45I | ...TT CGAA...
...AAGC TT... | Clostridium sporogenes | 60 oC |
| Csp6I | ...G TAC...
...CAT G... | Corynebacterium species | 65 oC |
| CspAI | ...A CCGGT...
...TGGCC A... | Corynebacterium species | 65 oC |
| CviAII | ...C ATG...
...GTA C... | Chlorella virus | 60 oC |
| CviJI | ...RG CY...
...YC GR... | Chlorella strain | 65 oC |
| DdeI | ...C TNAG...
...GANT C... | Desulfovibrio desulfuricans | 50 oC |
| DpnI | ...GmeA TC...
...CT meAG... | Streptococcus pneumoniae | 37 oC |
| DpnII | ...N GATCN...
...NCTAG N... | Streptococcus pneumoniae | 65 oC |
| DraI | ...TTT AAA...
...AAA TTT... | Deinococcus radiophilus | 50 oC |
| DraII | ...RG GNCCY...
...TCCNG GR... | Deinococcus radiophilus | 55 oC |
| DraIII | ...CACNNN GTG...
...GTG NNNCAC... | Deinococcus radiophilus | 60 oC |
| DrdI | ...GACNNNN NNGTC...
...CTGNN NNNNCAG... | Deinococcus radiophilus | 37 oC |
| DriI | ...GACNNN NNGTC...
...CTGNN NNNCAG... | Deinococcus radiophilus | 60 oC |
| DseDI | ...GACNNNN NNGTC...
...CTGNN NNNNCAG... | Deinococcus species | 60 oC |
| EaeI | ...Y GGCCR...
...RCCGG Y... | Enterobacter aerogenes | 65 oC |
| EagI | ...C GGCCG...
...GCCGG C... | Pantoea agglomerans | 55 oC |
| Eam1104I | ...CTCTTCN NNNN...
...GAGAAGNNNN N... | Enterobacter amnigenus | 60 oC |
| Eam1105I | ...GACNNN NNGTC...
...CTGNN NNNCAG... | Enterobacter amnigenus | 37 oC |
| EarI | ...CTCTTCN NNNN...
...GAGAAGNNNN N... | Enterobacter aerogenes | 60 oC |
| EciI | ...GGCGGANNNNNNNNNNN N...
...CCGCCTNNNNNNNNN NNN... | Escherichia coli | 55 oC |
| Ecl136II | ...GAG CTC...
...CTC GAG... | Enterobacter cloacae | 55 oC |
| EclHKI | ...GACNNN NNGTC...
...CTGNN NNNCAG... | Enterobacter cloacae | 50 oC |
| EclXI | ...C GGCCG...
...GCCGG C... | Enterobacter cloacae | 60 oC |
| Eco24I | ...GRGCY C...
...C TCGRG... | Escherichia coli | 60 oC |
| Eco31I | ...GGTCTCN NNNNN...
...CCAGAGNNNNN N... | Escherichia coli | 50 oC |
| Eco32I | ...GAT ATC...
...CTA TAG... | Escherichia coli | 37 oC |
| Eco47I | ...G GWCC...
...CCWG G... | Escherichia coli | 37 oC |
| Eco47III | ...AGC GCT...
...TCG CGA... | Escherichia coli | 37 oC |
| Eco52I | ...C GGCCG...
...GCCGG C... | Escherichia coli | 37 oC |
| Eco57I | ...CTGAAGNNNNNNNNNNNNNNNN N...
...GACTTCNNNNNNNNNNNNNN NNN... | Escherichia coli | 37 oC |
| Eco57MI | ...CTGRAGNNNNNNNNNNNNNNNN N...
...GACYTCNNNNNNNNNNNNNN NNN... | Escherichia coli | 37 oC |
| Eco72I | ...CAC GTG...
...GTG CAC... | Escherichia coli | 60 oC |
| Eco81I | ...CC TNAGG...
...GGANT CC... | Escherichia coli | 60 oC |
| Eco88I | ...C YCGRG...
...GRGCY C... | Escherichia coli | 37 oC |
| Eco91I | ...G GTNACC...
...CCANTG G... | Escherichia coli | 37 oC |
| Eco105I | ...TAC GTA...
...ATG CAT... | Escherichia coli | 37 oC |
| Eco130I | ...C CWWGG...
...GGWWC C... | Escherichia coli | 37 oC |
| Eco147I | ...AGG CCT...
...TCC GGA... | Escherichia coli | 37 oC |
| EcoICRI | ...GAG CTC...
...CTC GAG... | Escherichia coli | 37 oC |
| EcoNI | ...CCTNN NNNAGG...
...GGANNN NNTCC... | Escherichia coli | 37 oC |
| EcoO109I | ...RG GNCCY...
...YCCNG GR... | Escherichia coli | 37 oC |
| EcoO65I | ...G GTNACC...
...CCANTG G... | Escherichia coli | 37 oC |
| EcoP15I | ...CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN NNN...
...GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN N... | Escherichia coli | 37 oC |
| EcoRI | ...G AATTC...
...CTTAA G... | Escherichia coli | 37 oC |
| EcoRII | ...N CCWGGN...
...NGGWCC N... | Escherichia coli | 37 oC |
| EcoR V | ...GAT ATC...
...CTA TAG... | Escherichia coli | 37 oC |
| EcoT14I | ...C CWWGG...
...GGWWC C... | Escherichia coli | 37 oC |
| EcoT22I | ...ATGCA T...
...T ACGTA... | Escherichia coli | 37 oC |
| EcoT38I | ...GRGCY C...
...C YCGRG... | Escherichia coli | 37 oC |
| EgeI | ...GGC GCC...
...CCG CGG... | Enterobacter gergoviae | 37 oC |
| EheI | ...GGC GCC...
...CCG CGG... | Pantoea agglomerans | 37 oC |
| ErhI | ...C CWWGG...
...GGWWC C... | Erwinia rhapontici | 37 oC |
| Esp3I | ...CGTCTCN NNNNN...
...GCAGAGNNNNN N... | Erwinia species | 37 oC |
