| Enzyme | Restriction site | Origin | Temperature |
|---|
| Bst2BI | ...C ACGAG...
...GTGCT C... | Geobacillus stearothermophilus | 37 oC |
| Bst2UI | ...CC WGG...
...GGW CC... | Geobacillus stearothermophilus | 60 oC |
| Bst4CI | ...ACN GT...
...TG NCA... | Geobacillus stearothermophilus | 65 oC |
| Bst6I | ...CTCTTCN NNNN...
...GAGAAGNNNN N... | Geobacillus stearothermophilus | 65 oC |
| Bst98I | ...C TTAAG...
...GAATT C... | Geobacillus stearothermophilus | 37 oC |
| Bst1107I | ...GTA TAC...
...CAT ATG... | Geobacillus stearothermophilus | 37 oC |
| BstACI | ...GR CGYC...
...CTGC RG... | Geobacillus stearothermophilus | 37 oC |
| BstAPI | ...GCANNNN NTGC...
...CGTN NNNNACG... | Geobacillus stearothermophilus | 37 oC |
| BstAUI | ...T GTACA...
...ACATG T... | Geobacillus stearothermophilus | 37 oC |
| BstBI | ...TT CGAA...
...AAGC TT... | Geobacillus stearothermophilus | 65 oC |
| BstBAI | ...YAC GTR...
...RTG CAY... | Geobacillus stearothermophilus | 65 oC |
| BstC8I | ...GCN NGC...
...CGN NCG... | Geobacillus stearothermophilus | 55 oC |
| BstDSI | ...C CRYGG...
...GGYRC C... | Geobacillus stearothermophilus | 65 oC |
| BstDEI | ...C TNAG...
...GANT C... | Geobacillus stearothermophilus | 60 oC |
| BstEII | ...G GTNACC...
...CCANTG G... | Geobacillus stearothermophilus | 60 oC |
| BstENI | ...CCTNN NNNAGG...
...GGANNN NNTCC... | Geobacillus stearothermophilus | 65 oC |
| BstF5I | ...GGATGNN N...
...CCTAC NNN... | Geobacillus stearothermophilus | 65 oC |
| BstFNI | ...CG CG...
...GC GC... | Geobacillus stearothermophilus | 60 oC |
| BstH2I | ...RGCGC Y...
...Y CGCGR... | Geobacillus stearothermophilus | 65 oC |
| BstHHI | ...GCG C...
...C GCG... | Geobacillus stearothermophilus | 50 oC |
| BstKTI | ...GAT C...
...C TAG... | Geobacillus stearothermophilus | 37 oC |
| BstMBI | ...N GATCN...
...NCTAG N... | Geobacillus stearothermophilus | 65 oC |
| BstMCI | ...CGRY CG...
...GC YRGC... | Geobacillus stearothermophilus | 50 oC |
| BstMWI | ...GCNNNNN NNGC...
...CGNN NNNNNCG... | Geobacillus stearothermophilus | 55 oC |
| BstNI | ...CC WGG...
...GGW CC... | Geobacillus stearothermophilus | 60 oC |
| BstNSI | ...RCATG Y...
...Y GTACR... | Geobacillus stearothermophilus | 37 oC |
| BstOI | ...CC WGG...
...GGW CC... | Geobacillus stearothermophilus | 60 oC |
| BstPI | ...G GTNACC...
...CCANTG G... | Geobacillus stearothermophilus | 60 oC |
| BstPAI | ...GACNN NNGTC...
...CTGNN NNCAG... | Geobacillus stearothermophilus | 65 oC |
| BstSCI | ...N CCNGGN...
...NGGNCC N... | Geobacillus stearothermophilus | 55 oC |
| BstSFI | ...C TRYAG...
...GAYRT C... | Geobacillus stearothermophilus | 60 oC |
| BstSNI | ...TAC GTA...
...ATG CAT... | Geobacillus stearothermophilus | 37 oC |
| BstUI | ...CG CG...
...GC GC... | Geobacillus stearothermophilus | 60 oC |
| BstV1I | ...GCAGCNNNNNNNN NNNNN...
...CGTCGNNNNNNNNNNNN N... | Geobacillus stearothermophilus | 55 oC |
| BstV2I | ...GAAGACNN NNNNN...
...CTTCTGNNNNNN N... | Geobacillus stearothermophilus | 55 oC |
| BstXI | ...CCANNNNN NTGG...
...GGTN NNNNNACC... | Geobacillus stearothermophilus | 50 oC |
| BstX2I | ...R GATCY...
...TCTAG R... | Geobacillus stearothermophilus | 60 oC |
| BstYI | ...R GATCY...
...YCTAG R... | Geobacillus stearothermophilus | 60 oC |
| BstZI | ...C GGCCG...
...GCCGG C... | Geobacillus stearothermophilus | 50 oC |
| BstZ17I | ...GTA TAC...
...CAT ATG... | Geobacillus stearothermophilus | 37 oC |
| Bsu15I | ...AT CGAT...
...TAGC TA... | Bacillus subtilis | 37 oC |
| Bsu36I | ...CC TNAGG...
...GGANT CC... | Bacillus subtilis | 37 oC |
| BsuRI | ...GG CC...
...CC GG... | Bacillus subtilis | 37 oC |
| BsuTUI | ...AT CGAT...
...TAGC TA... | Bacillus subtilis | 37 oC |
| BtgI | ...C CRYGG...
...GGYRC C... | Geobacillus thermoglucosidasius | 37 oC |
| BtgZI | ...GCGATGNNNNNNNNNN NNNNN...
...CGCTACNNNNNNNNNNNNNN N... | Geobacillus thermoglucosidasius | 60 oC |
| BtrI | ...CAC GTC...
...GTG CAG... | Geobacillus stearothermophilus | 60 oC |
| BtsI | ...GCAGTGNN N...
...CGTCAC NNN... | Geobacillus thermoglucosidasius | 37 oC |
| BveI | ...ACCTGCNNNN NNNNN...
...TGGACGNNNNNNNN N... | Brevundimonas vesicularis | 37 oC |
