| Enzyme | Restriction site | Origin | Temperature |
|---|
| BalI | ...TGG CCA...
...ACC GGT... | Curtobacterium albidum | 37 oC |
| BamHI | ...G GATCC...
...CCTAG G... | Curtobacterium albidum | 37 oC |
| BanI | ...G GYRCC...
...CCRYG G... | Paenibacillus thiaminolyticus | 50 oC |
| BanII | ...GRGCY C...
...C YCGRG... | Paenibacillus thiaminolyticus | 37 oC |
| BauI | ...C ACGAG...
...GTGCT C... | Bacillus aquaemaris | 37 oC |
| BbeI | ...GGCGC C...
...C CGCGG... | Bifidobacterium breve | 37 oC |
| BbrPI | ...CAC GTG...
...GTG CAC... | Brevibacillus brevis | 37 oC |
| BbsI | ...GAAGACNN NNNNN...
...CTTCTGNNNNNN N... | Brevibacillus laterosporus | 37 oC |
| BbuI | ...GCATG C...
...C GTACG... | Bacillus species | 37 oC |
| BbvI | ...GCAGCNNNNNNNN NNNNN...
...CGTCGNNNNNNNNNNNN N... | Brevibacillus brevis | 37 oC |
| Bbv12I | ...GWGCW C...
...C WCGWG... | Brevibacillus brevis | 37 oC |
| BbvCI | ...CC TCAGC...
...GGAGT CG... | Brevibacillus brevis | 37 oC |
| BccI | ...CCATCNNNN NN...
...GGTAGNNNNN N... | Bacteroides caccae | 37 oC |
| BceAI | ...ACGGCNNNNNNNNNNNN NNN...
...TGCCGNNNNNNNNNNNNNN N... | Bacillus cereus | 37 oC |
| BciVI | ...GTATCCNNNNNN N...
...CATAGGNNNNN NN... | Bacillus circulans | 37 oC |
| BclI | ...T GATCA...
...ACTAG T... | Bacillus caldolyticus | 50 oC |
| BcnI | ...CC SGG...
...GGS CC... | Brevibacillus centrosporus | 37 oC |
| BcuI | ...A CTAGT...
...TGATC A... | Bacillus coagulans | 37 oC |
| BfaI | ...C TAG...
...GAT C... | Bacteroides fragilis | 37 oC |
| BfmI | ...C TRYAG...
...GAYRT C... | Bacillus firmus | 37 oC |
| BfrI | ...C TTAAG...
...GAATT C... | Bacteroides fragilis | 37 oC |
| BfrBI | ...ATG CAT...
...TAC GTA... | Bacteroides fragilis | 37 oC |
| BfuI | ...GTATCCNNNNNN N...
...CATAGGNNNNN NN... | Bacillus firmus | 37 oC |
| BfuAI | ...ACCTGCNNNN NNNNN...
...TGGACGNNNNNNNN N... | Bacillus fusiformis | 50 oC |
| BfuCI | ...N GATCN...
...NCTAG N... | Bacillus fusiformis | 37 oC |
| BglI | ...GCCNNNN NGGC...
...CGGN NNNNCCG... | Bacillus globigii | 37 oC |
| BglII | ...A GATCT...
...TCTAG A... | Bacillus globigii | 37 oC |
| BlfI | ...T CCGGA...
...AGGCC T... | Bacillus licheniformis | not known |
| BlnI | ...C CTAGG...
...GGATC C... | Brevibacterium linens | 37 oC |
| BlpI | ...GC TNAGC...
...CGANT CG... | Bacillus lentus | 37 oC |
| Bme1390I | ...CC NGG...
...GGN CC... | Bacillus megaterium | 37 oC |
| Bme1580I | ...GKGCM C...
...C MCGKG... | Bacillus megaterium | 37 oC |
| Bme18I | ...G GWCC...
...CCWG G... | Bacillus megaterium | 37 oC |
| BmgBI | ...CAC GTC...
...GTG CAG... | Bacillus megaterium | 37 oC |
| BmrI | ...ACTGGGNNNNN N...
...TGACCCNNNN NN... | Bacillus megaterium | 37 oC |
| BmuI | ...ACTGGGNNNNN N...
...TGACCCNNNN NN... | Bacillus megaterium | 37 oC |
| BmtI | ...GCTAG C...
...C GATCG... | Bacillus megaterium | 37 oC |
| BmyI | ...GDGCH C...
...C HCGDG... | Bacillus mycoides | 37 oC |
| BoxI | ...GACNN NNGTC...
...CTGNN NNCAG... | Microbacterium oxydans | 37 oC |
| BpcI | ...C TRYAG...
...GAYRT C... | Bacillus sphaericus | not known |
| BpiI | ...GAAGACNN NNNNN...
...CTTCTGNNNNNN N... | Bacillus pumilus | 37 oC |
| BpmI | ...CTGGAGNNNNNNNNNNNNNNNN N...
...GACCTCNNNNNNNNNNNNNN NNN... | Bacillus pumilus | 37 oC |
| BptI | ...CC WGG...
...GGW CC... | Virgibacillus pantothenticus | not known |
| Bpu10I | ...CC TNAGC...
...GGANT CG... | Bacillus pumilus | 37 oC |
| Bpu1102I | ...GC TNAGC...
...CGANT CG... | Bacillus pumilus | 37 oC |
| Bpu14I | ...TT CGAA...
...AAGC TT... | Bacillus pumilus | 37 oC |
| BpuAI | ...GAAGACNN NNNNN...
...CTTCTGNNNNNN N... | Bacillus pumilus | 37 oC |
| BpuEI | ...CTTGAGNNNNNNNNNNNNNNNN N...
...GAACTCNNNNNNNNNNNNNN NNN... | Bacillus pumilus | 25 oC |
