| Enzyme | Restriction site | Origin | Temperature |
|---|
| BsaI | ...GGTCTCN NNNNN...
...CCAGAGNNNNN N... | Geobacillus stearothermophilus | 50 oC |
| Bsa29I | ...AT CGAT...
...TAGC TA... | Geobacillus stearothermophilus | 37 oC |
| BsaAI | ...YAC GTR...
...RTG CAY... | Geobacillus stearothermophilus | 50 oC |
| BsaBI | ...GATNN NNATC...
...CTANN NNTAG... | Geobacillus stearothermophilus | 37 oC |
| BsaHI | ...GR CGYC...
...CYGC RG... | Geobacillus stearothermophilus | 37 oC |
| BsaJI | ...C CNNGG...
...GGNNC C... | Geobacillus stearothermophilus | 37 oC |
| BsaMI | ...GAATGCN N...
...CTTAC GNN... | Geobacillus stearothermophilus | 37 oC |
| BsaWI | ...W CCGGW...
...WGGCC W... | Geobacillus stearothermophilus | 37 oC |
| BsaXI | ...NNNN NNNNNNNNNACNNNNNCTCCNNNNNNNNNN N...
...N NNNNNNNNNNNNTGNNNNNGAGGNNNNNNN NNNN... | Geobacillus stearothermophilus | 37 oC |
| Bsc4I | ...CCNNNNN NNGG...
...GGNN NNNNNCC... | Bacillus schlegelii | 37 oC |
| Bse1I | ...ACTGGN N...
...TGAC CNN... | Geobacillus stearothermophilus | 37 oC |
| Bse118I | ...R CCGGY...
...TGGCC R... | Geobacillus stearothermophilus | 37 oC |
| Bse21I | ...CC TNAGG...
...GGANT CC... | Bacillus species | 37 oC |
| Bse3DI | ...GCAATGNN N...
...CGTTAC NNN... | Geobacillus stearothermophilus | 37 oC |
| Bse8I | ...GATNN NNATC...
...CTANN NNTAG... | Bacillus species | 37 oC |
| BseAI | ...T CCGGA...
...AGGCC T... | Geobacillus stearothermophilus | 50 oC |
| BseBI | ...CC WGG...
...GGW CC... | Geobacillus stearothermophilus | 37 oC |
| BseCI | ...AT CGAT...
...TAGC TA... | Geobacillus stearothermophilus | 37 oC |
| BseDI | ...C CNNGG...
...GGNNC C... | Geobacillus stearothermophilus | 37 oC |
| BseGI | ...GGATGNN N...
...CCTAC NNN... | Geobacillus stearothermophilus | 37 oC |
| BseJI | ...GATNN NNATC...
...CTANN NNTAG... | Geobacillus stearothermophilus | 37 oC |
| BseLI | ...CCNNNNN NNGG...
...GGNN NNNNNCC... | Geobacillus stearothermophilus | 37 oC |
| BseMI | ...GCAATGNN N...
...CGTTAC NNN... | Geobacillus stearothermophilus | 37 oC |
| BseMII | ...CTCAGNNNNNNNNNN N...
...GAGTCNNNNNNNN NNN... | Geobacillus stearothermophilus | 50 oC |
| BseNI | ...ACTGGN N...
...TGAC CNN... | Geobacillus stearothermophilus | 37 oC |
| BsePI | ...G CGCGC...
...CGCGC G... | Geobacillus stearothermophilus | 37 oC |
| BseRI | ...GAGGAGNNNNNNNNNN N...
...CTCCTCNNNNNNNN NNN... | Bacillus species | 37 oC |
| BseSI | ...GKGCM C...
...C MCGKG... | Geobacillus stearothermophilus | not known |
| BseXI | ...GCAGCNNNNNNNN NNNNN...
...CGTCGNNNNNNNNNNNN N... | Geobacillus stearothermophilus | 37 oC |
| BseX3I | ...C GGCCG...
...GCCGG C... | Geobacillus stearothermophilus | 37 oC |
| BseYI | ...C CCAGC...
...GGGTC G... | Bacillus species | 37 oC |
| BsgI | ...GTGCAGNNNNNNNNNNNNNNNN N...
...CACGTCNNNNNNNNNNNNNN NNN... | Bacillus sphaericus | 37 oC |
| Bsh1236I | ...CG CG...
...GC GC... | Bacillus sphaericus | 37 oC |
| Bsh1285I | ...CGRY CG...
...GC YRGC... | Bacillus sphaericus | 37 oC |
| BshFI | ..GG CC..
..CC GG.. | Bacillus sphaericus | 37 oC |
| BshNI | ...G GYRCC...
...CCRYG G... | Bacillus sphaericus | 37 oC |
| BshTI | ...A CCGGT...
...TGGCC A... | Bacillus sphaericus | 37 oC |
| BsiEI | ...CGRY CG...
...GC YRGC... | Bacillus species | 37 oC |
| BsiHKAI | ...GWGCW C...
...C WCGWG... | Geobacillus stearothermophilus | 37 oC |
| BsiHKCI | ...C YCGRG...
...GRGCY C... | Bacillus species | not known |
| BsiSI | ...C CGG...
...GGC C... | Geobacillus stearothermophilus | 37 oC |
| BsiWI | ...C GTACG...
...GCATG C... | Bacillus species | 37 oC |
| BsiYI | ...CCNNNNN NNGG...
...GGNN NNNNNCC... | Bacillus pumilus | not known |
| BslI | ...CCNNNNN NNGG...
...GGNN NNNNNCC... | Bacillus species | 37 oC |
| BslFI | ...GGGACNNNNNNNNNN N NNNNN...
...CCCTGNNNNNNNNNNNNNN N N... | Geobacillus stearothermophilus | 37 oC |
